Single Cell
Sequencing
Breakthroughs in the coming decades will transform the world. We accelerate this progress by powering fundamental research across the life sciences, including oncology, immunology, and neuroscience.
About
The analysis of individual cells allows researchers to gain new knowledge in a diverse set of research areas, including developmental biology, tumor heterogeneity and pathogenesis and disease progression. It allows the comparison of genomic / transcriptomes / epigenome of individual cells and allows the evaluation of similarities and differences within a population of cells. The 10x Genomics' Chromium Single Cell system using Diploide is the single cell profiling platform that allows the analysis of large numbers of cells with the highest capture efficiency (up to 65%). Its technology divides the reactions into nanoliter scale drops containing pearls with unique barcodes called GEM (Gel Bead-In EMulsions). This core technology can be used to divide individual cells or high molecular weight DNA to prepare next-generation sequencing libraries in parallel.
The single cell service using the Diploid Chromium 10xG platform allows for reliable investigation of the genomic, transcriptomic and epigenomic individual cells with minimal bias. We can offer complete solutions for:
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Single cell genomics (variation of copy number).
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Transcriptomic of individual cells (gene expression profile and immune profile of T / B cells).
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Epigenomics of individual cells (chromatin accessibility, ATAC-seq of individual cells).
How does it work.
Prepare the sample
Mail it to our lab
Postage is prepaid
Benefits
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Up to 80,000 cells analyzed per chip with up to 65% cell capture efficiency
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Highest throughput sequencing platforms, including the Illumina NovaSeq 6000, provide cost-effective single-cell solutions
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Samples accepted at different stages of the 10x workflow - cells, Gel Beads in Emulsion (GEMs), and 10x barcoded cDNA libraries
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*NEW* Dead cell removal improves data quality, especially for projects with suboptimal samples.
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Proprietary cell freezing protocol maintains cell viability during transit and provides a convenient method to ship samples
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Interactive analysis report provides an intuitive way to explore the data and find biological insights
Platform
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Identify and characterize rare cell types.
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Analyze and understand cellular heterogeneity and how this contributes to your biological system
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Perform cell phenotype with single cell RNA sequence to identify new targets, biomarkers, and cell types and states without the need for preselected targets
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Evaluate mRNA and cell surface protein expression profiles within the same cell
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Perform high-throughput, high-resolution functional genetic displays on tens of thousands of cells simultaneously
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Evaluate Complete Gene Expression Phenotypes for Individual CRISPR Disturbances.
SINGLE CELL
GENE EXPRESSION
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Discover cellular heterogeneity derived from epigenetic variability.
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Better understand the regulatory networks of genes that are upstream of gene expression
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Define cell types and states for lineage and development program monitoring.
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Accelerate biomarker discovery
SINGLE CELL
ATAC
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Reveal clonality, diversity, antigenic specificity, and cellular context.
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Assemble and annotate complete V (D) J genetic sequences
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Match single T cell α and β chain TCR sequences
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Match single B cell heavy and light chain immunoglobulin (Ig) sequences with full isotype resolution
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Simultaneously measures TCR, B cell Ig, cell surface protein expression, and 5 'gene expression in the same cells
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Link full-length paired α and β TCR chain sequences with TCR-pMHC specificity
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Simultaneously measures cell surface protein expression with gene expression
